Enzo Life Sciences的ROS-ID® NO Detection kit以紅色熒光的NO檢測試劑為主要成分，可通過熒光顯微鏡直接實時監(jiān)測活細胞中NO的產生。這種非熒光的、可滲透細胞的NO檢測染料在O2存在的情況下能夠與NO發(fā)生反應，具有高特異性、靈敏性和準確性，產生不溶于水的紅色熒光產物。這種染料對過氧亞硝酸鹽沒有反應性，因此可以區(qū)分過氧亞硝酸鹽和一氧化氮。

ROS-ID® NO Detection kit中的熒光探針對活性氯或溴不敏感，因此不用于檢測這些分析物。染色后，該染料產生的熒光產物可通過熒光顯微鏡進行觀察。當需要同時檢測額外的熒光信號（綠色或橙色）時，建議使用650/670 nm的組合。ROS-ID® NO Detection kit包含足夠的試劑，可對活細胞（貼壁或懸?。┻M行至少200次檢測。

產品特色

通過熒光顯微鏡直接實時監(jiān)測活體細胞中NO的產生

高特異性、靈敏性和準確性，產生不溶于水的紅色熒光產物

可區(qū)分過氧亞硝酸鹽和一氧化氮

試劑盒內包含一氧化氮誘導劑和清除劑

產品信息

部分產品引用文獻

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7. Anti-inflammatory effects of olive-derived hydroxytyrosol on lipopolysaccharide-induced inflammation in RAW264.7 cells: Y. Yonezawa, et al.; J. Vet. Med. Sci. 80, 1801 (2018)

8. Oligodendrocyte RasG12V expressed in its endogenous locus disrupts myelin structure through increased MAPK, nitric oxide, and notch signaling: H.E. Titus, et al.; Glia 65, 1990 (2017)

9. Improved arterial flow-mediated dilation after exertion involves hydrogen peroxide in overweight and obese adults following aerobic exercise training: A.T. Robinson, et al.; J. Hypertens. 34, 1309 (2016)

10. STAT3 Represses Nitric Oxide Synthesis in Human Macrophages upon Mycobacterium tuberculosis Infection: C.J. Queval, et al.; Sci. Rep. 6, 29297 (2016)

11. Flavonoids activate endothelial nitric oxide synthase by altering their phosphorylation via mitogen-activated protein kinase pathways in glucose-induced endothelial cells: C.E. Kim, et al.; J. Funct. Foods 17, 676 (2015)

12. Ginsenoside Rg3 regulates S-Nitrosylation of the NLRP3 inflammasome via suppression of iNOS: S. J. Yoon, et al.; Biochem. Biophys. Res. Commun. 463, 1184 (2015)

13. Propofol restores TRPV1 sensitivity via a TRPA1-, nitric oxide synthase-dependent activation of PKCε: P. Sinharoy, et al.; Pharmacol. Res. Perspect. 3, e00153 (2015)

14. Reduced flow-and acetylcholine-induced dilations in visceral compared to subcutaneous adipose arterioles in human morbid obesity: I. Grizelj, et al.; Microcirculation 22, 44 (2015)

15. Anti-inflammatory effects of Edaravone and Scutellarin in activated microglia in experimentally induced ischemia injury in rats and in BV-2 microglia: Y. Yuan, et al.; BMC Neurosci. 15, 125 (2014)

16. Lipopolysaccharide (LPS) stimulation of fungal secondary metabolism: Z.G. Khalil, et al.; Mycology 5, 168 (2014)

17. Cyclosporine attenuates arginine transport, in human endothelial cells, through modulation of cationic amino acid transporter-1: A. Grupper, et al.; Am. J. Nephrol. 37, 613 (2013)

18. Identification and characterization of a functional mitochondrial angiotensin system: P.M. Abadir, et al.; PNAS 108, 14849 (2011)

19. Loss of bcl-2 during the senescence exacerbates the impaired angiogenic functions in endothelial cells by deteriorating the mitochondrial redox state: M. Uraoka, et al.; Hypertension 58, 254 (2011)

詳情請聯(lián)系Enzo Life Sciences金牌代理——欣博盛生物